quantitative g6pd kit trinity g-6-pdh kit  (Trinity Biotech)

Journal: Malaria Journal

Article Title: Molecular characterization of G6PD mutations reveals the high frequency of G6PD Aures in the Lao Theung population

doi: 10.1186/s12936-020-03560-7

Figure Lengend Snippet: Identification of G6PD Aures by an ARMS-PCR assay. a Schematic representation of the primer location and predicted size of PCR products for the G6PD Aures and internal control fragment. b Agarose gel electrophoresis represents band of G6PD Aures (226 bp) and internal control fragments (519 bp). Lane 1 represents the 100 bp DNA marker. Lanes 2–4 represent G6PD Aures-positive samples, and lanes 5–6, G6PD Aures-negative samples

Article Snippet: All samples were measured for G6PD enzyme activity in duplicate using a quantitative G6PD kit (Trinity G-6-PDH Kit, Trinity Biotech, Bray, Ireland).

Techniques: Control, Agarose Gel Electrophoresis, Marker

Journal: Malaria Journal

Article Title: Molecular characterization of G6PD mutations reveals the high frequency of G6PD Aures in the Lao Theung population

doi: 10.1186/s12936-020-03560-7

Figure Lengend Snippet: Agarose gel electrophoresis representing 8 G6PD Asian genotypes. Lane 1 represents a 100 bp DNA marker. Lane 2 is a G6PD mutation standard marker. Lanes 3–7 are PCR products of unknown samples, lane 8 is the normal control, and lane 9 is the mutant positive control

Article Snippet: All samples were measured for G6PD enzyme activity in duplicate using a quantitative G6PD kit (Trinity G-6-PDH Kit, Trinity Biotech, Bray, Ireland).

Techniques: Agarose Gel Electrophoresis, Marker, Mutagenesis, Control, Positive Control

Journal: Malaria Journal

Article Title: Molecular characterization of G6PD mutations reveals the high frequency of G6PD Aures in the Lao Theung population

doi: 10.1186/s12936-020-03560-7

Figure Lengend Snippet: Prevalence and frequency of G6PD gene mutations in the Lao Theung ethnic group

Article Snippet: All samples were measured for G6PD enzyme activity in duplicate using a quantitative G6PD kit (Trinity G-6-PDH Kit, Trinity Biotech, Bray, Ireland).

Techniques:

Journal: Malaria Journal

Article Title: Molecular characterization of G6PD mutations reveals the high frequency of G6PD Aures in the Lao Theung population

doi: 10.1186/s12936-020-03560-7

Figure Lengend Snippet: The histogram shows the correlation between G6PD activity (U/gHb) and G6PD mutations in the Lao Theung population. a G6PD activity (U/gHb) in each G6PD mutation group for males. Enzyme activity below 2.0 U/gHb indicates G6PD deficiency; enzyme activity of 2.0 to 4.67 U/gHb indicates G6PD intermediate. All hemizygous subjects presented G6PD deficiency. b G6PD activity for the female population. Homozygous subjects presented G6PD deficiency, while the heterozygous subjects have intermediate to normal G6PD enzyme activity

Article Snippet: All samples were measured for G6PD enzyme activity in duplicate using a quantitative G6PD kit (Trinity G-6-PDH Kit, Trinity Biotech, Bray, Ireland).

Techniques: Activity Assay, Mutagenesis

Journal: Malaria Journal

Article Title: Molecular characterization of G6PD mutations reveals the high frequency of G6PD Aures in the Lao Theung population

doi: 10.1186/s12936-020-03560-7

Figure Lengend Snippet: Prevalence and molecular characteristics of G6PD deficiency in Lao PDR

Article Snippet: All samples were measured for G6PD enzyme activity in duplicate using a quantitative G6PD kit (Trinity G-6-PDH Kit, Trinity Biotech, Bray, Ireland).

Techniques: Mutagenesis

Journal: The American Journal of Tropical Medicine and Hygiene

Article Title: Screening for Glucose-6-Phosphate Dehydrogenase Deficiency Using Three Detection Methods: A Cross-Sectional Survey in Southwestern Uganda

doi: 10.4269/ajtmh.16-0552

Figure Lengend Snippet: Demographic characteristics of children surveyed *

Article Snippet: G6PDd prevalence was determined using three detection methods: a quantitative G6PD enzyme activity assay (Trinity Biotech ® G-6-PDH kit), a qualitative point-of-care test (CareStart TM G6PD rapid diagnostic test [RDT]), and molecular detection of the G6PD A− G202A allele.

Techniques: Microscopy, Infection, Mutagenesis

Journal: The American Journal of Tropical Medicine and Hygiene

Article Title: Screening for Glucose-6-Phosphate Dehydrogenase Deficiency Using Three Detection Methods: A Cross-Sectional Survey in Southwestern Uganda

doi: 10.4269/ajtmh.16-0552

Figure Lengend Snippet: Distribution of enzymatic activity by G202A genotype and CareStart™ G6PD rapid diagnostic test (RDT). Gray points represent individuals who tested deficient by CareStart™ G6PD RDT. Dotted line at 60% enzymatic activity represents the cut-off point between normal and mild G6PD deficiency as defined by the World Health Organization. Dotted line at 30% shows recent proposed cut-off to denote “deficient” males and females.8 Hemi = hemizygous; Het = heterozygous; Homo = homozygous; WT = wild-type.

Article Snippet: G6PDd prevalence was determined using three detection methods: a quantitative G6PD enzyme activity assay (Trinity Biotech ® G-6-PDH kit), a qualitative point-of-care test (CareStart TM G6PD rapid diagnostic test [RDT]), and molecular detection of the G6PD A− G202A allele.

Techniques: Activity Assay, Diagnostic Assay

Journal: The American Journal of Tropical Medicine and Hygiene

Article Title: Screening for Glucose-6-Phosphate Dehydrogenase Deficiency Using Three Detection Methods: A Cross-Sectional Survey in Southwestern Uganda

doi: 10.4269/ajtmh.16-0552

Figure Lengend Snippet: Diagnostic performance of CareStart ™ G6PD RDT at different cut-off points of enzymatic activity

Article Snippet: G6PDd prevalence was determined using three detection methods: a quantitative G6PD enzyme activity assay (Trinity Biotech ® G-6-PDH kit), a qualitative point-of-care test (CareStart TM G6PD rapid diagnostic test [RDT]), and molecular detection of the G6PD A− G202A allele.

Techniques: Diagnostic Assay, Activity Assay